Chronic ROS exposure by long-term ethanol feeding induces mild steatosis and slightly elevation of serum ALT, with little or no liver inflammation.32 Moreover, aging is closely associated with oxidative stress, and aged mice showed reduction of stress tolerance, lower GSH/GSSG ratio and higher MDA levels in the liver compared with young mice; however, serum ALT level in aged mice was not differ from young mice.33 In our data, ROS was weakly or not increased by Con A injection in the liver and spleen of WT mice. The gene discussed is GPT; the disease is steatosis.