Double immunofluorescent staining of NOTCH3 and keratin in this coculture model showed that bundles of NOTCH3-positive NHDFs surrounded keratin-positive HO1-N-1 cancer nests (Fig 2B), which resembled the distribution of NOTCH3-positive fibroblasts around the cancer nests in the human OSCC samples. The gene discussed is HMOX1; the disease is cancer.