To further confirm the role of CAV-1 in the antioxidation of cholesterol-induced hypercholesterolemia, for the Nrf2-Keap1-GCLC pathway, the subcellular localization of Nrf2, Keap1, and GCLC was quantified in isolated renal tissue through immunohistochemistry and immunoblot analysis. Here, CAV1 is linked to familial hypercholesterolemia.