To further confirm the role of CAV-1 in the antioxidation of cholesterol-induced hypercholesterolemia, for the Nrf2-Keap1-GCLC pathway, the subcellular localization of Nrf2, Keap1, and GCLC was quantified in isolated renal tissue through immunohistochemistry and immunoblot analysis. The gene discussed is KEAP1; the disease is familial hypercholesterolemia.