We also assessed the mRNA expression of HSC-sourced growth factors including α-SMA, TGF-β and TIMP-1 in HSCs, and the concentration of liver fibrosis markers including LN, HA and Collagen IV in the supernatants with or without IL-22 (20, 40 ng/mL) for 48h or 72h. Here, TIMP1 is linked to Hepatic fibrosis.