In this study we found that miR186 was downregulated inmalignant PCa cell lines and clinical PCa specimens, and we experimentally demonstrated that miR186 maintained the epithelial phenotype and reduced migration and invasion, soft-agar colony formation, vasculogenic mimicry (VM) formation capability of human PCa cells through targeting Twist1, suggesting that miR186 might serve as a tumor-suppressive miRNA in the development and progression of PCa. This evidence concerns the gene TWIST1 and posterior cortical atrophy.