We added an inhibitor of anoikis, the Rho-associated protein kinase-1 (ROCK1) inhibitor Y-27632, to early passage ccRCC cell lines before typical trypsin passaging and noted a significant increase in clonogenic frequency, suggesting that ccRCC cells are sensitive to anoikis in vitro (Fig. 5A). The gene discussed is ROCK1; the disease is nonpapillary renal cell carcinoma.