In order to determine the role of PAK1 in cell proliferation and viability, we constructed the cell lines stably expressing wild-type PAK1 (WT), constitutively active PAK1 mutant (PAK1-CA, PAK1T423E), dominant-negative PAK1 mutant (PAK1-DN, PAK1H83,86L, K299R) [19, 20], or GFP using a neuroblastoma cell line, SH-SY5Y cell line, lentiviral vector, and fluorescence-activated cell sorting. Here, PAK1 is linked to neuroblastoma.