To confirm that the HEL-specific B cells were mounting a CD4+ T cell dependent response to subcutaneous tumor, we inoculated mice s.c. with B16.mHELMCC cells, adoptively transferred 1×106 naïve SWHELRag2−/− B cells and 2×105 naive 5C.C7 Rag2−/− CD4+ T cells on day 3 and characterized splenic B cells by flow cytometry 13, 30 and 71 days later. Here, CD4 is linked to neoplasm.