B7-H3 expression in human tumor cells was assessed by FACS analysis including lung cancer (A549-luc and NCIH460-luc), breast cancer (MDA-MB231-luc), colorectal cancer (HT-29-luc), pancreatic cancer (BXPC3-luc), cervix cancer (Hela-luc), prostate cancer (PC-3M-luc) and glioblastoma (U87MG-luc). The gene discussed is CD276; the disease is prostate cancer.