In our study we took advantage of the ATS1-KO mice to determine the contribution of stromal Adamts1 in the metastatic cascade and, in addition, we included tumor cells with down-regulated Adamts1. We performed intravenous tail injection of control B16F1 and modified B16-shAts1 and B16-shSCR melanoma cells used in previous sections. This evidence concerns the gene COL4A5 and neoplasm.