Direct comparison of the two marker patterns revealed identity of the SERPINA1‐derived peptide SERPINA1[Thr363‐Phe376], whereas the COL1A1‐, B2M‐, and FGA‐derived AKI peptide markers in MALDI are for the most part smaller fragments of the previously described CE‐MS‐identified peptide markers. Here, COL1A1 is linked to acute kidney injury.