The presence of the three activating hot-spot mutations (E542K, E545K and H1047R) in exons 9 and 20 of the PIK3CA gene and the oncogenic E17K mutation in the PH domain of the AKT1 gene was analyzed in laryngeal and hypopharyngeal squamous cell carcinoma tissue specimens by mutant-enriched sequencing (Supplementary Figure S1). The gene discussed is PIK3CA; the disease is squamous cell carcinoma.