To examine whether the conversion of primary CLL cells by GIFT4 treatment could lead to the gain-of-function immune-stimulatory activities of GIFT4-CLL cells on autologous T cells, we stimulated the PBMC from CLL patients with GIFT4, GM-CSF and IL-4, or PBS for 5 days. The gene discussed is CSF2; the disease is B-cell chronic lymphocytic leukemia.