FBXW7 and colorectal carcinoma: In vitro, 5-FU can trigger apoptosis in CRC cells and it appeared to be initiated by caspase-dependent death pathway.33,34 To investigate the role of FBXW7 mutation in drug resistance, we examined the induction of growth inhibition in a dose-dependent manner using FBXW7 knockout HCT116 (HCT116FBXW7(−/−)) and DLD-1 (DLD-1FBXW7(−/−)),23,24 in parallel with FBXW7 wild-type (FBXW7(+/+)) counterpart cell lines (Figure 1a and Supplementary Figure S1a).