HES1 and head and neck squamous cell carcinoma: As shown in Fig. S3A both siRNA may reduce NOTCH1 and HES1 after 24 h transfection, and both 5 μM and 10 μM DAPT may decrease NOTCH1 and HES1 expression after 24 h treatment in human HNSCC cell line CAL27 and FaDu.