Depletion of Nup153 added an additional block to HIV-1 infection on top of T5Cyp-mediated restriction (Additional file 5: Fig. S5) but, notably, resulted in a greater rescue of infection relative to control DsRed KD cells [50] (Fig. 6e, Additional file 5: Figure S5). This evidence concerns the gene NUP153 and HIV-1 infection.