SMPD1 and melanoma: Pharmacological inhibition of A-SMase by amitriptyline (5 μM, 1 h before cisplatin treatment), a widely used lysosomotropic molecule that induces loss of A-SMase [43], significantly decreased the percentage of cisplatin-induced B16-W6_scr apoptotic cells at levels comparable to B16-W6_pSIL10 cells (Figure 2C), further indicating that low A-SMase inhibits cisplatin-induced melanoma cell death.