Consistent with the inhibitory role of A-SMase in melanoma progression [37, 38] B16-F1_B1A tumours grew more slowly than B16-W6_pSIL10 tumours (Figure 5B) and exhibited significant lower formation of lipidated LC3 (Figure 5C), and up-regulated levels of p62 (Figure 5C) and phospho-Akt/S6 (Figure 5D). Here, SMPD1 is linked to melanoma.