HIC1 and carcinoma: To examine whether HIC1 is inactive by hypermethylation in NSCLC, we examined the methylation status of HIC1 promoter in cell lines and 10 pairs of NSCLC carcinoma and para-carcinoma tissues by methylation specific PCR (MSP) and bisulfite sequencing PCR (BSP) (Figure 1A, 1B and 1C).