After implantation and inoculation of RM9-Luc-pIRES-KLK3 cells in the prostate only (Figure 3A) for mice orthotopic prostate cancer model and after the inoculation of cells into prostate and tail vein for mice orthotopic prostate cancer with metastatic model, micrometastatic deposits were detected by real-time bioluminescent imaging 7 days after transplantation; lung metastases spread exponentially on dynamic observation in the metastatic model (Figure 4A). The gene discussed is KLK3; the disease is prostate cancer.