We predicted that 6 genes including ANXA1 would be regulated by miR196a using 4 prediction softwares (TargetScan, MiRanda, Diana MicroT and PicTar), and confirmed the reduction of these genes (ANXA1, HOXA7, HOXB7, ING5, CDC73 and SMURF1) by miR-196a in breast cancer cells using qPCR analysis. Here, ANXA1 is linked to breast cancer.