RUNX2 and melanoma: In order to define potential mediators of the RUNX2-mediated effects on migration and invasion, we generated 250 μg of proteins from stable 1205LU melanoma cell lines expressing two types of RUNX2 ShRNA; ShRUNX2-2 (targeting RUNX2 3′UTR) and ShRUNX2-3 (targeting the RUNX2 coding sequence) as previously described [14].