Among the 25 genes with an inverse correlation between methylation and expression were H19, GSTM1, GSTP1, G0S2.[105] These findings are consistent with results showing reduced expression of H19 in ACC as a function of loss of maternal allele with the duplication of paternal allele, leading to biallelic expression of IGF2 gene and decrease in H19 and p57kip2 expression.[45, 50] Also, ACC cells lines were treated with the cytosine methylation inhibitor 5-aza-2′-deoxycytine as an attempt to reduce the methylation of H19 promoter and thereby increase its expression. This evidence concerns the gene IGF2 and adrenal cortex carcinoma.