A human esophagus squamous cell carcinoma cell line with short hairpin RNA (shRNA)-mediated DS-epi1 loss-of-function (Thelin et al., 2012) and cultured aortic smooth muscle cells from Dse−/− mice (Bartolini et al., 2013) both have a reduced level of plasma membrane protrusions and an abnormal actin cytoskeleton, which changes their migration properties similar to the findings in CNC cells. This evidence concerns the gene DSE and esophageal squamous cell carcinoma.