Taken together, this results show that downregulation of p-AKT and p-p65 by deguelin takes place in the live fraction of cells, where only a minority of cells stain positive forAnnexin V (S3 Fig), indicating that downregulation of AKT/NFκB by deguelin precedes the onset of apoptosis, and thus strongly suggesting that deguelin induces apoptosis in CLL cells by downregulation of the AKT/NFκB pathway. Here, AKT1 is linked to B-cell chronic lymphocytic leukemia.