PKM and neoplasm: While the decrease of plectin from N_0 to N_8 by the HeLa cells is likely for the purpose of maintaining proteostasis, the sudden increase of plectin in N_12 indicates an alleviated release of exosome,[37] agreeing the starvation behavior cancer cells that attempt to manipulate tumor microenvironments (i.e., stimulate stromal and endothelial cells) to aid their progression.[38,39] There is another interesting observation: while the amount of all other proteins decrease in a certain degree in at least one sample of nanonets, the amount of pyruvate kinase (PKM) gradually increases over time.