Among those candidates, we picked up 4 genes such as Axin2, Regucalcin, Lect2 and GS. To confirm that those genes are regulated by CTNNB1 mutation in human HCC, we established stable cell line expressing mutant β-catenin (S33Y) using Hep3B cell line. The gene discussed is AXIN2; the disease is hepatocellular carcinoma.