Last but not least, our above pathway enrichment analysis of target proteins and previous researches both demonstrated that SND exerted its cardiotonic effect by regulation of TNF signaling pathway33, Apoptosis35, Hypertrophic cardiomyopathy (HCM)22 and Dilated cardiomyopathy23, 35, and TNF existed in above four pathways simultaneously. This evidence concerns the gene TNF and hypertrophic cardiomyopathy.