To further illustrate whether miR-138 affects human osteosarcoma cell proliferation, apoptosis and invasion through DEC2, DEC2 overexpression plasmid was transfected into the U2OS cells in the presence of miR-138 mimic, whereas DEC2 siRNA was transfected into the MG-63 cells in the presence of miR-138 inhibitor. Here, BHLHE41 is linked to osteosarcoma.