To determine whether SFN requires Nrf2 to impair HIV infection of macrophages, we depleted primary hMDMs of Nrf2 with siRNA and assessed the impact of this treatment on infection with VSV-G-pseudotyped HIV-1 luciferase reporter virus, both in the presence and absence of 10 μM SFN. Here, NFE2L2 is linked to HIV infectious disease.