In cell level, MSI2 protein and mRNA levels were higher in Capan-2, PANC-1 and BxPC-3 cells than that in SW1990 and AsPC-1 cells, which was completely contrary to the protein and mRNA levels of Numb in these five PC cell lines (Figure 2c–2e). This evidence concerns the gene MSI2 and pachyonychia congenita.