However, our results demonstrated that DNA methylation may play a key role to silence CNR1 in UM-UC-3 cell line, since after demethylation treatment the expression of CNR1 was activated regardless of the exon change in EZH2. In addition, another 9 genes marked with PcG and reported to be hypermethylated in cancer showed significant changes throughout the demethylation treatment (Supplementary Fig. S3)40. Here, EZH2 is linked to cancer.