As a key cytoskeleton regulator, Cdc42 is known to regulate the actin cytoskeleton to promote filopodia formation.16, 17 We have reported that Cdc42 is significantly downregulated in TRCs compared with control melanoma cells (the cells on 2D rigid plastic).3 To investigate the relationship between Cdc42 and YAP, we knocked down Cdc42 in the cells on 2D rigid plastic. The gene discussed is CDC42; the disease is melanoma.