Silencing Nupr1 in 2D melanoma cells elevated the number of mice with tumors (from 9 out of 16 mice to 12 out of 16 mice); overexpressing Nupr1 in TRCs significantly suppressed the number of mice with tumors (from 15 out of 16 mice to 9 out of 16 mice) (Table 1). This evidence concerns the gene NUPR1 and melanoma.