To further explore the role of Nupr1 in vivo, we either silenced Nupr1 in melanoma cells grown on 2D rigid plastic (whose Nupr1 levels were high; Figure 1a; Supplementary Figure 1a) or overexpressed Nupr1 in TRCs (whose Nupr1 levels were low; Figure 1a; Supplementary Figure 1a) and injected those treated cells into mice at 2000 cells per mouse subcutaneously. This evidence concerns the gene NUPR1 and melanoma.