Previously established CASP7 promoter,30 a kind gift from Professor Srikumar P Chellappan (H Lee Moffitt Cancer Center and Research Institute, FL, USA) and a 622 base pair (–622/+1) CASP7 promoter subcloned into pGL3 luciferase vector termed as P-2350 and P-622, respectively, were subjected to dual luciferase assay after 48 h of co-transfecting the promoter constructs with ERα (gift from Professor Ratna K Vadlamudi, University of Texas Health Science Center, San Antonio, TX, USA) in MCF7 cells. The gene discussed is CASP7; the disease is cancer.