In our previous animal study, we showed that depletion of macrophages by clodrolip, inhibition of high-mobility-group box chromosomal protein-1 (HMGB1) through a specific antibody [9], or reduction of inflammation through activation of α7 nicotinic acetylcholine receptor [10] reduce stroke injury of mice that were subjected to tibia fracture one day after stroke. The gene discussed is HMGB1; the disease is Stroke.