SHH and neoplasm: To validate whether the SHH-driven reciprocal signaling axis regulates de novo tumor cell protein turnover, PSC+DDCM.tub-KDEL and PDA+LyrM37-KDEL CTAP cells were differentially isotopically labeled, treated with a SHH inhibitor or vehicle, and cell-specific proteomes were quantified in heteroculture (Figure 5D).