Finally, we developed a 3D organotypic co-culture model, including ECM Matrigel substrate, CAFs, and mesothelial and tumor cells labeled discriminately from bottom to the top layer, which faithfully represents the histologic and biologic microenvironment of ovarian cancer peritoneal metastasis, to evaluate the effects of MARCKS inhibition in CAF effects on tumor cells adhesion and invasion (Figure 6C). Here, MARCKS is linked to neoplasm.