First, stably transfected HCC cell lines (MHCC97H-sh-control-Luc, MHCC97H-sh-SIRT1-Luc, MHCC97H-sh-SIRT1-LV-PGC-1α-Luc) were established to achieve the lentivirus-mediated knockdown of SIRT1 and overexpression of PGC-1α. Here, PPARGC1A is linked to hepatocellular carcinoma.