First, in our study, we detected SIRT1 and PGC-1α levels in HCC samples simutaneously, and observed that SIRT1 overexpression was highly associated with the upregulation of PGC-1α in HCC tumors compared to adjacent nontumoral liver tissues (r = 0.569, P < 0.001) (Figure 6A1, 6A2). This evidence concerns the gene PPARGC1A and hepatocellular carcinoma.