Moreover, the ectopic expression of PGC-1α in SIRT1-depleted MHCC97H cells restored mitochondrial biogenesis and ATP production, which further confirmed that SIRT1 facilitates HCC invasion by activating the SIRT1/PGC-1α axis to increase mitochondrial biogenesis. The gene discussed is PPARGC1A; the disease is hepatocellular carcinoma.