Tumor suppressor roles of PRSS8 were supported by in vitro study by transfection of PRSS8 overexpressing plasmid, showing that increased expression of PRSS8 led to the upregulation of P21 and E-cadherin and to the downregulation of Cyclin D1, Snail and Twist, which are well associated with cell proliferation and epithelial mesenchymal transition. The gene discussed is TWIST1; the disease is neoplasm.