To determine the biological roles of PRSS8 in ESCC cells, particularly the significance of PRSS8 promoter methylation, we treated KYSE450 and EC9706 cells with 100μM DAC or DAC+siR-PRSS8 for 24 and 48 hours, and determined cell proliferation, motility and migration using MTT, wound healing and transwell methods. Here, PRSS8 is linked to esophageal squamous cell carcinoma.