After 72 h of infection with recombinant replication-deficient adenoviruses co-expressing ZBTB20 and EGFP (hereafter Ad-ZBTB20) at the MOI of 400, more than 85% of GH3 cells were green fluorescent protein-positive by flow cytometry assay (Supplementary Fig. 13). This evidence concerns the gene ZBTB20 and infection.