In this study, we performed a quantitative DNA methylation analysis by pyrosequencing in Brazilian patients at HCC, cirrhotic, and non-cirrhotic stages, and assessed the relationship between methylation levels and clinicopathological characteristics, with the aim of examining the relevance of the hypermethylation of RASSF1A and DOK1 as a molecular marker of HCC. Here, DOK1 is linked to hepatocellular carcinoma.