We then found calpain inhibitor III (10 mg/kg) pretreatment or Ad-shcalpain2 (2 × 1011vp/mice) infection could reverse the reduction of both Atg3 and Atg7 in fatty livers at 6 h of reperfusion (Figures 6b and c and Supplementary Figures 6a and b). This evidence concerns the gene ATG7 and infection.