In addition, although A674563 potently inhibits FLT3-ITD activity in the biochemical assays, FLT3-ITD auto-phosphorylation in the isogenic BaF3 cells as well the downstream target Stat5's phosphorylation in the established AML cell lines MV4-11, it does not potently inhibit FLT3-ITD's auto-phosphorylation in the MV4-11 cells until 5 μM, which indicates that there might be some hidden mechanisms regarding to the FLT3-ITD's auto-phosphorylation and requires further detailed elucidation. The gene discussed is FLT3; the disease is acute myeloid leukemia.