In LN229 and U87MG cells, the HM20 and HM40 treatments decreased the activation of PI3K, Akt and Akt-associated cell cycle-related proteins, such as cyclin D1 and cyclin A. In addition, Hono-Mag combination treatment inactivated CDK4, CDK6, Akt and decreased the proliferation through cell cycle arresting at the G0/G1 phase in GBM cells. The gene discussed is CDK4; the disease is glioblastoma.