To confirm the different phenotypes of hepatocytes isolated, we performed Immunohistochemistry and western blotting to evaluate the expression of HCC markers at 1st passage in-vitro culture (Fig 4) including Vimentin, CK18, ALB, ARG1 β-Catenin, c-Met and Id-1. The gene discussed is ARG1; the disease is hepatocellular carcinoma.