Because connective tissue cells were visible during cultivation, we measured HGF, FGF2, SDF-1, and PDGF compared to stable chordoma cell lines (MUG-Chor118 and U-CH1-319) and healthy normal skin fibroblasts (fibro), as well as tumor-surrounding cancer cells (TSC). The gene discussed is FGF2; the disease is tuberous sclerosis.