Genetic manipulation includes phosphatase and tensin homolog (PTEN) knock-out NASH-HCC mouse model [39], argumenter of liver regeneration (ALR)-knock-out mouse model, knock-out of melanocortin 4 receptor (MC4R) [40] and the 129S1/SvImJ strain of mice with a metabolic iron abnormality [41]. The gene discussed is MC4R; the disease is hepatocellular carcinoma.