It was demonstrated that feeding mice choline/folate-deficient diet resulted in their developing histopathologic features of NASH, and there exists a close correlation between the extent of liver injury and the abnormal expression of iron metabolic genes, such as transferrin receptor, ferritin heavy chain, solute carrier family 40 (iron-regulated transporter), member 1 (Slc40a1, Fpn1) and their related proteins, and pronounced down-regulation of the iron regulatory protein 1 (IRP1). This evidence concerns the gene ACO1 and metabolic dysfunction-associated steatohepatitis.