Analysis of the enrichment of classes of the LOF mutations for the hGIS2 genes in ovarian cancer revealed that deletions (includes frameshift deletions; P<0.0001), insertions (includes frameshift insertions; P<0.0001), frameshift deletions (P<0.0001), frameshift insertions (P<0.0001) and nonsense mutations (P=0.0015) were present at significantly increased levels; many but not all of these LOF mutations were in BRCA1 or BRCA2 (Supplementary Data 3). This evidence concerns the gene BRCA1 and ovarian cancer.