KMT2A and acute myeloid leukemia: To assess the role of Rheb1 in AML, we first examined Rheb1 expression in the BM of wild-type (WT) mice and established MLL-AF9-induced AML mice by Western blotting analysis and found that Rheb1 protein was overexpressed in AML mice in comparison with WT mice (Additional file 1: Figure S2A).