As expected, the phosphorylation levels of S6 and 4E-BP1 in GFP+ cells and corresponding subpopulations (including K+G−, K+G+, and K−G+ cells) were significantly decreased in Rheb1Δ/Δ AML cells compared with the control (Fig. 5a–d and Additional file 1: Figure S3H and I). Here, EIF4EBP1 is linked to acute myeloid leukemia.