BM GFP+ cells were sorted and cultured, and the apoptosis status was examined at 72 h after culture through flow cytometry using 7-AAD and annexin V. The percentage of apoptotic GFP+ cells (annexin V+) in the BM was increased (Fig. 4g), and the proportions of apoptotic K+G−, K+G+, and K−G+ cells (annexin V+) were also significantly increased in Rheb1-deficient AML cells compared with the control (Fig. 4h). The gene discussed is ANXA5; the disease is acute myeloid leukemia.