We provided evidence that dasatinib promotes cellular apoptosis through inhibition of Akt/mTOR activities, and prevents exosomal release through downregulation of beclin-1 and Vps34 -dependent autophagic activity, indicating distinct dasatinib-induced mechanisms of apoptotic response and exosomes release in imatinib-resistant CML cells. Here, AKT1 is linked to chronic myelogenous leukemia, BCR-ABL1 positive.