Moreover, it is of particular interest to investigate whether some known lupus-related environmental factors such as endocrine disruptor chemicals and lupus-inducing drugs will affect DNA methylation at DLK1-Dio3 domain, especially during critical and vulnerable developmental stages, to induce abnormal DLK1-Dio3 miRNAs expression and autoimmunity. The gene discussed is DIO3; the disease is systemic lupus erythematosus.