To understand whether altered DNA methylation contributes to the upregulation of genomic imprinted DLK1-Dio3 miRNAs in lupus splenic cells, we measured the global DNA methylation levels in splenocytes, purified splenic CD4+ T cell, CD19+ B cells, and splenic CD4-CD19- cells from MRL and MRL-lpr mice. Here, DLK1 is linked to systemic lupus erythematosus.